Statistical Optimization of Citric Acid Production using Cladosporium sp Isolated from Sathuragiri Hills

Research Article

Statistical Optimization of Citric Acid Production using Cladosporium sp Isolated from Sathuragiri Hills

T.Sivakumar, T.Angaladevi, K.Nirmaladevi, B.Muthulakshmi, T.Shankar

Corresponding Author : T.Sivakumar

Post Graduate and Research Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, India.

Email ID : sivasadhana@yahoo.co.in

Received : 2014-04-02 Accepted : 2014-04-22 Published : 2014-04-22

Abstract : Response surface methodology (RSM) was used to optimize the cultivation conditions for citric acid production, 30 experimental runs with different combinations of four factors and five levels were carried out. The variables used for the factorial analysis were pH, temperature, glucose and ammonium chloride, named X1, X2, X3 and X4 this design, respectively. The effects of the four independent variables on citric acid production and the experimental response along with the predicted response obtained from the regression equation for each run are shown. It can be seen that, there was a considerable variation in the citric acid production depending on the four chosen variables. The maximum citric acid production (17.3 g/L) was achieved. This adequately indicated that choosing appropriate cultivation conditions could evidently enhance the yield of citric acid.

Keywords : Citric acid, RSM, CCD,Cladosporium sp, Optimization

Citation : T.Sivakumar et al (2014) Statistical Optimization of Citric Acid Production using Cladosporium sp Isolated from Sathuragiri Hills. J. of Advanced Botany and Zoology, V1I2. DOI : 10.5281/zenodo.890218

Copyright : © 2014 T.Sivakumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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