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Stainless Steel Sponge as a Carrier for Immobilization of Rhizopus nigricans Isolate used for Biotrnsformation of Progesterone to 11α-hydroxprogesterone

HomeStainless Steel Sponge as a Carrier for Immobilization of Rhizopus nigricans Isolate used for Biotrnsformation of Progesterone to 11α-hydroxprogesterone

Research Article

Stainless Steel Sponge as a Carrier for Immobilization of Rhizopus nigricans Isolate used for Biotrnsformation of Progesterone to 11α-hydroxprogesterone

Essam M. Ahmed and Mohamed I. Ammar

Corresponding Author : Essam. M. Ahmed,

Chemistry of Natural and Microbial Products, National Research Center, Dokki, Cairo, Egypt, P.O.12622.,

Email ID : eahmed98@hotmail.com

Received : 2015-03-08 Accepted : 2015-05-08 Published : 2015-05-08

Abstract : Biotransformation is an important tool to provide the intermediate for steroid manufacturing. Stainless steel sponge was used as a carrier to immobilize an isolate of Rhizopus nigricans which can convert progesterone to 11α-hydroxyprogesterone (11α-HP). The conversion ability of the isolate was measured. The immobilized cells showed the ability to withstand the stress of the biotransformation process. Different parameters were studied to achieve the optimum conditions for 11α-HP production. The immobilized cells were reused for seven successive runs and maintained high bioconversion abilities during the first two runs then lost the conversion ability in seventh run.

Keywords : Biotransformation, Rhizopus nigricans, 11α- hydroxyprogesterone

Citation : Essam M. Ahmed et al. (2015). Stainless Steel Sponge as a Carrier for Immobilization of Rhizopus nigricans Isolate used for Biotrnsformation of Progesterone to 11α-hydroxprogesterone. J. of Advancement in Medical and Life Sciences. V3I1.03 DOI : 10.5281/zenodo.1000364

Copyright : © 2015 Essam M. Ahmed. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Journal of Advancement in Medical and Life Sciences

ISSN : 2348-294X

Volume 3 / Issue 1

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