Production of Transgenic Miniature Piglets by Transfer of ifn-γ Gene Transfected Somatic Cells into Enucleated Oocytes
Suo Li, Jin-Dan Kang, Jun-Xue Jin, Cheng-Du Cui,Wen-Xue Li, Qing-Shan Gao,Yu Hong, Hai-Ying Zhu, Long Jin and Xi-Jun Yin
Corresponding Author : Xi-Jun Yin
Department of Animal Science, Agricultural College of Yanbian University.
Email ID : firstname.lastname@example.org
Received : 2014-01-10 Accepted : 2014-01-22 Published : 2014-01-22
Abstract : Generation of transgenic pigs by somatic cell nuclear transfer (SCNT) has provided a new avenue to modify disease resistance or susceptibility in pigs. In this study, we evaluated the efficiency of producing cloned transgenic piglets by SCNT. Donor cells were isolated from a 2-year-old female Wuzhishan miniature piglet. Cells transfected with either the swine broad-spectrum anti-viral interferon-Î³ gene (IFN-Î³) and non-transfected cells between passages 3 and 5,were used for cloning. We examined the developmental potential of porcine nuclear transfer (NT) embryos when IFN-Î³ and non-transfected cells were used as donor cells.There was no significant difference in cleavage and development rates to the blastocyst stage for IFN-Î³ NT embryos compared with non-transgenic donor cells (78.4% and 10.8% vs. 80.6% and 16.2%, respectively).Cloned embryos reconstructed with IFN-Î³ gene were introduced into 4 surrogate mothers and 1 of these gave birth to 7 cloned piglets. Genomic DNA from cloned piglets was screened, and 5 PCR-positive founders were identified. The control group were introduced into 8 surrogate mothers. Of these mothers, 5 became pregnant as detected by ultrasonography on days 24â€“26 after embryo transfer. 3 surrogate mothers gave birth to 18 cloned piglets by eutocia after 114â€“117 days of gestation. While the number of piglets surviving to adulthood was not significantly different between transgenic and non-transgenic donor cells (57.1% vs. 66.6%). We successed to product transfer of IFN-Î³ gene Wuzhishan inbred miniature pigs by somatic cell nuclear transfer, to provide a suitable approach to produce resistant transgenic pigs. The disease resistance of IFN-Î³ transgenic pigs has yet to be further study.
Keywords : transgenic pig, somatic cell nuclear transfer, ifn-γ
Citation : Xi-Jun Yin (2014) Production of Transgenic Miniature Piglets By Transfer of ifn-γ Gene Transfected Somatic Cells into Enucleated Oocytes . j. advanced botany and zoology.V1-I1. DOI : 10.5281/zenodo.1000089
Copyright : © 2014 Xi-Jun Yin, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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