Production of Transgenic Miniature Piglets by Transfer of ifn-γ Gene Transfected Somatic Cells into Enucleated Oocytes

Research Article

Production of Transgenic Miniature Piglets by Transfer of ifn-γ Gene Transfected Somatic Cells into Enucleated Oocytes

Suo Li, Jin-Dan Kang, Jun-Xue Jin, Cheng-Du Cui,Wen-Xue Li, Qing-Shan Gao,Yu Hong, Hai-Ying Zhu, Long Jin and Xi-Jun Yin

Corresponding Author : Xi-Jun Yin

Department of Animal Science, Agricultural College of Yanbian University.

Email ID : yinxj33@msn.com

Received : 2014-01-10 Accepted : 2014-01-22 Published : 2014-01-22

Abstract : Generation of transgenic pigs by somatic cell nuclear transfer (SCNT) has provided a new avenue to modify disease resistance or susceptibility in pigs. In this study, we evaluated the efficiency of producing cloned transgenic piglets by SCNT. Donor cells were isolated from a 2-year-old female Wuzhishan miniature piglet. Cells transfected with either the swine broad-spectrum anti-viral interferon-γ gene (IFN-γ) and non-transfected cells between passages 3 and 5,were used for cloning. We examined the developmental potential of porcine nuclear transfer (NT) embryos when IFN-γ and non-transfected cells were used as donor cells.There was no significant difference in cleavage and development rates to the blastocyst stage for IFN-γ NT embryos compared with non-transgenic donor cells (78.4% and 10.8% vs. 80.6% and 16.2%, respectively).Cloned embryos reconstructed with IFN-γ gene were introduced into 4 surrogate mothers and 1 of these gave birth to 7 cloned piglets. Genomic DNA from cloned piglets was screened, and 5 PCR-positive founders were identified. The control group were introduced into 8 surrogate mothers. Of these mothers, 5 became pregnant as detected by ultrasonography on days 24–26 after embryo transfer. 3 surrogate mothers gave birth to 18 cloned piglets by eutocia after 114–117 days of gestation. While the number of piglets surviving to adulthood was not significantly different between transgenic and non-transgenic donor cells (57.1% vs. 66.6%). We successed to product transfer of IFN-γ gene Wuzhishan inbred miniature pigs by somatic cell nuclear transfer, to provide a suitable approach to produce resistant transgenic pigs. The disease resistance of IFN-γ transgenic pigs has yet to be further study.

Keywords : transgenic pig, somatic cell nuclear transfer, ifn-γ

Citation : Xi-Jun Yin (2014) Production of Transgenic Miniature Piglets By Transfer of ifn-γ Gene Transfected Somatic Cells into Enucleated Oocytes . j. advanced botany and zoology.V1I102. DOI : 10.5281/zenodo.1000089

Copyright : © 2014 Xi-Jun Yin, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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