Plotting Phylogenetic Tree Using MOLEBLAST; Technical Guide

Research Article

Plotting Phylogenetic Tree Using MOLEBLAST; Technical Guide

Adebule, Adeniyi Philip

Corresponding Author : Adebule, Adeniyi Philip

Department of Microbiology, Ekiti State University, P.M.B. 5363, Ado Ekiti, Nigeria.

Email ID : philbule@yahoo.com

Received : 2018-01-28 Accepted : 2018-03-15 Published : 2018-03-15

Abstract : The quest for neighbour sequence of the query for plotting the phylogenetic tree above led to the use of MOLE-BLAST. The construction of phylogenetic tree was demonstrated using three (3) organisms with the accession numbers; NR_074540.1, NR_025685.1 and NR_117706.1 obtained from food waste contaminated soil in Ekiti State, Nigeria and identified as Bacillus cereus ATCC strain, Achromobacter insotitus strain and Achromobacter insuavis strain respectively. These organisms were seen to show some level of relatedness based on the tree diagram. The study guide for the construction phylogenetic tree exposes the easy approach that is needed to utilize the MOLE-BLAST for neighbour search for submitted sequence queries.

Keywords : Phylogenetic Tree, MOLEBLAST, BLAST, Sequence Analysis, Genetics

Citation : Adebule, Adeniyi Philip (2018). Plotting Phylogenetic Tree Using MOLEBLAST; Technical Guide, DR Congo. J. of Advancement in Medical and Life Sciences. V6I3-03. DOI : 10.5281/zenodo.1198928

Copyright : © 2018 Adebule, Adeniyi Philip . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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