Optimization of Invertase production using Saccharomyces cerevisiae MTCC 170 under varying cultural conditions

Research Article

Optimization of Invertase production using Saccharomyces cerevisiae MTCC 170 under varying cultural conditions

T. Sivakumar, P. Thangamathi, A. Mariashobana, T. Rathimeena, T. Shankar

Corresponding Author : T. Shankar,

Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi-626124, Tamil Nadu, India.

Email ID : ewcellulase@gmail.com

Received : 2014-03-02 Accepted : 2014-03-25 Published : 2014-03-25

Abstract : A Saccharomyces cerevisiae MTCC 170 was obtained from MTCC. The effect of different production parameters such as pH, temperature, incubation time, carbon source, nitrogen source (organic and inorganic), inoculum concentrations, sucrose concentrations, metal ions, surfactants, amino acids, buffers, agricultural residues and agricultural residue concentration on invertase production by the isolated Saccharomyces cerevisiae MTCC 170 strain were studied. The enzyme production was assayed in submerged fermentation (SmF). Maximum invertase activity was found at pH 6, 30°C, 48 hours, sucrose, yeast extract (organic nitrogen), ammonium chloride (inorganic nitrogen), 2% inoculum concentration, 2% sucrose concentration, calcium chloride, poly ethylene glycol, methionine, citrate buffer and orange peel - 4%. A higher titre of invertase enzyme activity (0.43 ± 0.005 IU/ml) was obtained in the optimized production medium.

Keywords : invertase, optimization, Saccharomyces cerevisiae MTCC 170, SmF, SSF.

Citation : T. Shankar , et al (2014) Optimization of Invertase production using Saccharomyces cerevisiae MTCC 170 under varying cultural conditions. J. of Advancement in Medical and Life Sciences. V1I2.01 DOI : 10.5281/zenodo.999363

Copyright : © 2014 T. Shankar . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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