Molecular Characterization of Lactic Acid Bacteria Isolated from locally produced ‘nono’ in owo lga, Ondo State.Nigeria.

Research Article

Molecular Characterization of Lactic Acid Bacteria Isolated from locally produced ‘nono’ in owo lga, Ondo State.Nigeria

Ojo R.O, Ibiyemi, D.A, Olatunde, A.O

Corresponding Author : Ojo R.O

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Received : 2020-08-25 Accepted : 2020-09-29 Published : 2020-09-29

Abstract : Lactic acid bacteria (LAB) were isolated from locally produced ‘nono’ samples from three different locations in Owo, Ondo State (South Western Nigeria). Microbiological analysis was carried out using serial dilution and pour plate method for the isolation. The Agar used was De Mann Roggosa Agar (MRS) Oxoid, Nutrient Agar and Nutrient Broth (Oxoid). Four (4) species of (LAB) were mostly present in the “Nono”: Lactococcus lactis, Lactobacillus plantarum, Lactobacillus casei and Enterococcus faecalis. Further identifaction at the species level indicated that all the lactococci isolates were L. lactis while three isolates of the Enterococci were Enterococci faecalis and lactobacilli were identified as Lactobacillus plantinum (1 Isolate). Lactobacillus casei (2 Isolates). PCR – RFLP method which is based on the amplification of 16s rRNA – ITS genes was used for the molecular characterization.

Keywords : “Nono”, Lactic Acid bacteria, molecular characterization

Citation : Ojo R.O. et al. (2018). Molecular Characterization of Lactic Acid Bacteria Isolated from locally produced ‘nono’ in owo lga, Ondo State.Nigeria.. J. of Advancement in Medical and Life Sciences. V8I1.05. DOI : 10.5281/zenodo.4071562

Copyright : © 2020 Ojo R.O. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Reviewer Comments : REVIEWER 1
The title of the paper is specific. The authors used keywords that match the work.
The authors need to include the molecular characterization in the introduction. The paper is approved for publishing sequel to corrections stated above.
Recommendation: The paper is hereby strongly recommended for publication.
Professor C.R. Falegan
Department of Microbiology
Ekiti State University, Ado – Ekiti
Ekiti State Nigeria.

REVIEWER 2
The paper’s title is okay. The abstract and the words also correlate with the work. The authors need to correct some grammatical errors and also beef up the introduction by including the molecular characterization into it
Recommendation: The paper is recommended for publication.
Prof. Hassan, A.O.
Department of Medical Laboratory Science.
Achievers University, Owo. Ondo State.


REVIEWER 3
The authors work is very apt and vividly explained the molecular aspect of Biology which is more acceptable today in the field of Biotechnology.
Recommendation: The paper is recommended for publication.

Dr. Temikotan, T.
Department of Biological Sciences,
Achievers University, Owo.
Ondo State. Nigeria.