ITS-Region Analysis for Medicinal Plants Identification

Research Article

ITS-Region Analysis for Medicinal Plants Identification

Pallavi Sahare

Corresponding Author : pallavi sahare

Department of Biochemistry, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India.

Email ID : pallavi.sahare@gmail.com

Received : 2014-08-02 Accepted : 2014-09-06 Published : 2014-09-06

Abstract : The ITS (Internal Transcribed Spacer) region in nuclear ribosomal DNA can be used not only for species identification but also for taxonomic clarification. The advantage for using this region is that it is a small sequence 600-1000bp in length (vary from species to species) and can be used even from a less amount of sample. The other advantage is that it is present in multiple copies so that after PCR (Polymerase Chain Reaction) produce sufficient concentration for further downstream processing together with purification, Restriction digestion, RAPD-RFLP analysis, sequencing, etc. Therefore this region sequencing can be used for authentic identification of medicinal plants. Further restriction analysis will be helpful in solving the ambiguity. This paper deals with the use of bioinformatics tool for restriction analysis of ITS-region sequencing data.

Keywords : Medicinal plants; ITS region; PCR, Plant identification.

Citation : Pallavi Sahare (2014) ITS-Region Analysis for Medicinal Plants Identification. J. of Advanced Botany and Zoology. V2I101. DOI : 10.5281/zenodo.999862

Copyright : © 2014 Pallavi Sahare. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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