Evolutionary Analysis of Regulatory Sequences in Promoter of Senecence-induced Glutamine Synthetase cytosolic isozyme GS1;5 in Arabidopsis thaliana
Mamoona Rauf. Muhammad Arif, Aziz Ud Din, Saima Rauf, Muhammad Waqar Hameed, Hussan Ara Begum
Corresponding Author : Mamoona Rauf,
Department of Botany, Garden Campus, Abdul Wali Khan University Mardan, 23200. Pakistan.
Email ID : firstname.lastname@example.org
Received : 2016-02-12 Accepted : 2016-06-27 Published : 2016-06-27
Abstract : Glutamine synthetase (GS; EC 220.127.116.11, L-glutamate: ammonia ligase ADP-forming) catalyzes the ATP-dependent addition of ammonium (NH4 +) to the Î³-carboxyl group of glutamate and produces glutamine. The enzyme is the product of multiple genes with complex promoters that ensure the transcriptional regulation of the genes in an organâ€ and tissueâ€specific manner and in response to a number of environmental variables affecting the nutritional status of the plant cell. In the present study we tested for the presence of highly conserved non-coding sequences (CNSs) in the 1000-bp promoter region by analyzing upstream promoter sequences (counted from the translation initiation codon), of Glutamine Synthetase (GS1;5) and its orthologous genes. A comparative genome-wide bioinformatic analysis performed for identification of evolutionarily preserved regulatory sequences has revealed highly conserved upstream non-coding sequences (CNSs) within 1000-bp promoter region by analyzing upstream promoter sequences (counted from the translation initiation codon), of GS1;5 from Arabidopsis thaliana and its orthologous genes in various plant species. Two consensus sequences are predicted by sequence logo at the position of -880 bp to -890 bp (CNS1) and -522 bp to -549 bp (CNS2) of Arabidopsis promoter region counted from the ATG. Thus identified putative cis-regulatory elements in the promoter region of GS1;5 are expected to allow physical binding of upstream regulatory proteins which are yet to be known, but controlling the senescence-dependent expression of this gene at promoter level in major plant species, under influence of environmental cues to initiate nitrogen remobilization from source to sink.
Keywords : glutamine synthetase, Conserved Regulatory Sequences, Orthologues
Citation : Mamoona Rauf et al. (2016). Evolutionary Analysis of Regulatory Sequences in Promoter of Senecence-induced Glutamine Synthetase cytosolic isozyme GS1;5 in . J. of Advancement in Medical and Life Sciences. V4I3 DOI : 10.5281/zenodo.1000257
Copyright : © 2016 Mamoona Rauf. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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