Evaluation and Optimization of a Method for Production of Methandienone Using PTFE Immobilized Fungal Cells
E. M. ahmed, M. I. Ammar
Corresponding Author : E. M. ahmed,
Chemistry of Natural and Microbial Products, National Research Center, Dokki, Cairo, Egypt, P.O.12622.
Email ID : email@example.com
Received : 2013-12-24 Accepted : 2014-01-23 Published : 2014-01-23
Abstract : A cell immobilization technique using polytetrafluoroethylene (PTFE) was employed in biotransformation process. Conversion of methyltestosterone to methandienone was achieved using the immobilized cells. An optimization strategy included two levels factorial model and three levels model was used. The most significant factors, glucose concentration, shaking speed and reaction time, were determined after Plakett-Burman screening study. Thereafter, the three levels quadratic model determined the optimum measures for thses significant factors and predicted the maximum product yield. The optimization led to improvement of the methandienone yield, from 45 % to 85% using the immobilized cells. The immobilized cells were reused for six runs with a good conversion outcomes and retained its metabolic activities after storage at 4ÂºC for one month. To the best of our knowledge, no previous work on this topic, the optimization and production of methandienone using the immobilized cells. The isolated fungus, Circinella mucoroides, was not previously used in similar work.
Keywords : Biotransformation, immobilization, PTFE, methandienone.
Citation : E. M. ahmed and M. I. Ammar (2015). Evaluation and optimization of a method for production of methandienone using PTFE immobilized fungal cells. J. of Bioprocessing and Chemical Engineering. V2I3. DOI : 10.5281/zenodo.893523
Copyright : © 2014 E. M. ahmed and M. I. Ammar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Volume / Issue
ScienceQ Publishing GroupDownload Article