Estimation of β-lactamase activity from multidrug resistant uropathological bacteria
Pallavi Sahare, Archana Moon
Corresponding Author : Archana Moon
Department of Biochemistry, RTM Nagpur University, Nagpur, India - 440033.
Email ID : firstname.lastname@example.org
Received : 2014-06-19 Accepted : 2014-07-12 Published : 2014-07-12
Abstract : The urinary tract infection (UTI) is a bacterial infection of the urinary system in both community and healthcare settings. β lactam antibiotics are the typical treatment option for UTI. But the increased rates of antibiotic resistance in bacteria have posed challenges in selecting the antibacterial agent. One of the mechanism bacteria developed to surpass the effect of β lactam antibiotics is the production and secretion of β-lactamases (EC 220.127.116.11). These antibiotics have a common element in their molecular structure, a four-atom ring known as the β-lactam ring. The β-lactamases breaks this ring open, deactivating the molecules antibacterial property. We have isolated and characterized the bacteria from UTI patients. The isolated bacteria were investigated for the antibiotic susceptibility towards commonly prescribed UTI antibiotics along with β-lactam antibiotics and isolated the multidrug resistant (MDR) clinical isolates. The secretary β-lactamase activities have been checked against eleven β-lactam antibiotics as a substrate by iodometric assay. This method depends upon the reduction of iodine by the hydrolyzed substrate.
Keywords : Î²-lactamase, iodometric method, urinary tract infection, multi drug resistance
Citation : Pallavi Sahare and Archana Moon (2014). Estimation of β-lactamase activity from multidrug resistant uropathological bacteria. J. of Advancement in Medical and Life Sciences. V1I4. DOI : 10.5281/zenodo.893561
Copyright : © 2014 Archana Moon. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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