Callogenesis Induction on different types of explants of Tetracera rosiflora Gilg.

Research Article

Callogenesis Induction on different types of explants of Tetracera rosiflora Gilg.

Muanyishay Lukanda Christophe, Diamuini Ndofunsu Aimé, Mutwale Kapepula Paulin, Ngombe Kabamba Nadège, Bulubulu Freddy, Luhahi Lumba Fatuma, Gédéon Bongo Ngiala, Luyindula Ndiku Sebastien, Pius T Mpiana

Corresponding Author : Pius T. Mpiana

Faculty of Sciences, University of Kinshasa, B.P. 190 Kinshasa XI, Kinshasa, Democratic Republic of Congo

Email ID : ptmpiana@gmail.com

Received : 2018-06-19 Accepted : 2018-08-28 Published : 2018-08-28

Abstract : Tetracera rosiflora Gilg is a medicinal plant used in the Congolese pharmacopoeia for the treatment of various diseases such as diabetes, arthritis, dysentery, hepatitis etc. It represents an attic of active compounds, which have multiple pharmacological properties. The aim of the current research is to induce callus formation in T. rosiflora Gilg. Henceforth, it is interesting to apply biotechnology tools to plants such as this specie in order to improve their chemical composition in bioactive explants. Our findings showed that a high percentage of calli (80%) was obtained with stems without nodes in the M4 medium and the stems with nodes gave neoformed buds. Obtained calli: friable whitish, whitish compact, whitish nodular, friable greenish and brittle brownish, were be produced according to the types of explants and the combination of growth regulators. The highest mass of fresh matter obtained was 92 mg in M4 medium and the lowest was 50 mg in M1 medium. The different combinations of growth regulators tested allowed the different explants to express their callogenic potentiality. However, the high proliferation rates have been obtained in the presence of 2,4-D that induced greater callogenesis of stems compared to leaves of T. rosiflora. The developed callogenesis system of Tetracera rosiflora is efficient and could give the possibility of production of the large scale of bioactive compound.

Keywords : Callogenesis, Tetracera rosiflora, growth regulators

Citation : Pius T. Mpiana. et al. (2018). Callogenesis Induction on different types of explants of Tetracera rosiflora Gilg.. J. of Advancement in Medical and Life Sciences. V7I1.02. DOI : 10.5281/zenodo.1407786

Copyright : © 2018 Pius T. Mpiana. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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