Assessment of the in-vitro activity and in-vivo preventive efficacy of phyto-fabricated silver nanoparticles and silver nanoparticle/mancozeb combination against Fusarium wilt of Lycopersicon esculentum mill
Godfrey O. Oribhabor , Solomon E. Omonigho
Corresponding Author : Godfrey O. Oribhabor
Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, Edo State, Nigeria.
Email ID : email@example.com
Received : 2020-08-22 Accepted : 2020-09-29 Published : 2020-09-29
Abstract : The antifungal activity of phyto-fabricated silver nanoparticle (AgNP) alone and in combination with the fungicide, mancozeb, against F. oxysporum f. sp. lycopersici were investigated in-vitro and under field trial. Treatments were applied at different concentrations (5.0, 7.5, 10.0, 12.5 %) while media without the treatments served as control. AgNP alone demonstrated an insignificant mycelial radial growth inhibitory effect (p > 0.05) on the fungus compared to the control. The mean radii of mycelial growth inhibition for AgNP alone ranged from 24.67 ± 0.33 to 25.33 ± 1.20 mm while the control was 25.67 ± 0.33 mm. However, AgNP/Mancozeb (50 % v/v) demonstrated significant inhibitory activity (p < 0.05) on the fungus compared to the control with a marked percentage inhibition ranging from 51.00 to 74.98 %. The fungal flora and physico-chemical parameters of the experimental plot were analyzed before sowing and soil-drench method was used to introduce the treatments in the field study at different concentrations (25, 50 and 75 mg/mL) while plants inoculated with the pathogen alone served as control. After 7, 14 and 21 Days Post Inoculation (DPI), disease incidence for plants treated with AgNP alone and AgNP/Mancozeb was highest at 50 mg/mL (44.60 ± 5.40 % and 39.93 ± 6.64 % respectively) at 21 DPI compared to control (29.30 ± 4.00 %). This study has demonstrated the optimization of ineffective large-sized AgNP by pairing with mancozeb and maximizing their synergistic effect against F. oxysporum f. sp. lycopercisi.
Keywords : Mancozeb, Nanoparticle, Phytopathogens, Phyto-fabricate, Characterization, Fusarium oxysporum,
Citation : Godfrey O. Oribhabor. et al. (2018). Assessment of the in-vitro activity and in-vivo preventive efficacy of phyto-fabricated silver nanoparticles and silver nanoparticle/mancozeb combination against Fusarium wilt of Lycopersicon esculentum mill. J. of Advancement in Medical and Life Sciences. V8I1.04. DOI : 10.5281/zenodo.4071540
Copyright : © 2020 Godfrey O. Oribhabor . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Journal of Advanced Botany and Zoology
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Reviewer Comments : Reviewer Comments #1
The paper title is okay. The aim of the paper is also achieved. The methodology displayed is precise and adequate and it correlates with the work discussed in the paper.
Recommendation: This paper is hereby strongly recommended for publication.
Department of Petroleum Engineering,
University of Benin,
Reviewer Comments #2
The paper is well articulated with standard methodologies applied. This research article is relevant to current and future contributing authors in the field of plant pathology and nanotechnology.
Recommendation: This paper is highly supported for publication.
Evbuomwan Nosa Oghoreye,
Department of Microbiology,
University of Benin