Assessment of Copper Caused Stress in Terms of Morphometric, Pigmental, Biochemical and Enzymatic Characteristics of Vigna Unguiculata (L)

Research Article

Assessment of Copper Caused Stress in Terms of Morphometric, Pigmental, Biochemical and Enzymatic Characteristics of Vigna Unguiculata (L)

M.Duraipandian, R.Sevugaperumal, and V.Ramasubramanian

Corresponding Author : V.Ramasubramanian

Department of Botany, Ayya Nadar Janaki Ammal College (Autonomous) Sivakasi 626124, India.

Email ID : drvrams@gmail.com

Received : 2015-06-01 Accepted : 2015-07-09 Published : 2015-07-09

Abstract : Metal contamination issues are common in india and elsewhere, in the cases of metal toxicity in mining industries, coal-burning power plants, foundries and smelters. Copper is an essential nutrient but in excess concentration responsible for environmental pollution. The excess of copper is released from many industries to cause soil and water pollution. The copper is a heavy metal highly accumulated in soil by anthropogenic activities. In the present study, the seedlings of (cow pea) Vigna unguiculata (L) walp were treated with various concentrations of copper chloride and its impact on the morphometric, pigmental, biochemical and enzymatic characters were studied. After ten days of treatment with different concentrations of copper chloride (2mM, 4mM, 6mM, 8mM, & 10 mM), the growth parameters such as leaf area, fresh weight, dry weight, shoot length, root length were found decreased than in the control. Pigmental characters such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoides content were decreased with the increase in the concentrations of copper chloride, but the content of anthocyanin was increased and the biochemical parameters such as soluble sugar and protein content were found decreased with the increase in the concentrations of copper chloride. But the contents of free amino acid, proline and leaf nitrate were increased with increase in the concentrations of copper chloride. The activities of enzyme such as catalase and peroxidase were found increased with the increase in the concentration of copper chloride. But the NRA enzyme activity was decreased. The Atomic Absorption of Spectroscopy (AAS) technique was employed to confirm the presence of copper chloride in treated plants and control. Comparison of the values of treated plants with control reveals that copper chloride has seriously affected the cow pea plants.

Keywords : Vigna Unguiculata (L), Copper Caused Stress, Metal contamination, metal toxicity

Citation : V.Ramasubramanian et al. (2015). Assessment of Copper Caused Stress in Terms of Morphometric, Pigmental, Biochemical and Enzymatic Characteristics of Vigna Unguiculata (L). J. of Advanced Botany and Zoology, V3I207. DOI : 10.5281/zenodo.999323

Copyright : © 2015 V.Ramasubramanian . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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