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%20%20%20%20Plasmid%20Profile%20of%20Antimicrobial%20Resistant%20Bacteria%20Isolated%20from%20Carrot%20(daucus%20carota%20l.%20Subsp.%20%20%20Sativus)%20in%20some%20selected%20markets%20in%20Ekiti%20State.%20Nigeria
Research article
  

Plasmid Profile of Antimicrobial Resistant Bacteria Isolated from Carrot (daucus carota l. Subsp. Sativus) in some selected markets in Ekiti State. Nigeria


Falegan, Christopher Rotimi., Ogunmoroti, Oluwole Samuel, Akoja, Sunday Olatunji and Adebule Philip

Affiliation


Akoja Sunday O,
Tel: 08061287909,
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Received: April 12, 2017,   Accepted: May 24, 2017,   Published:


Abstract:

Carrots are root vegetables and one of the important parts of human diet over the years. High nutrient values of the carrot (Dascus carota L. subsp sativus) and other extrinsic factors have created access for various antibiotic resistant pathogens. The aim of this study was to determine the prevalence of antibiotic resistance of bacteria isolated from carrots collected in five different locations in Ekiti-State. In this study, the microbial analysis of the carrot samples was done through poured plate method. The microbial load of the samples showed the mean bacteria count was 2.94×105 CFU/g and the mean total coliform count was 1.88 ×105 CFU/g, with a total of 48 bacteria isolated. Microbial characterization was carried out using cultural, biochemical and molecular techniques. The identified bacteria were Escherichia coli 18(37.5%), Enterobacter aerogens 11(20.8%), Klebsiella pneumoniae 8(16.7%), Bacillus cereus 6(14.6%), Proteus vulgaris 4(8.3%) and Seretia mascescens with 1(2.1%). Susceptibility to antibiotics was done using disc diffusion method. Plasmid detection for 15 isolates with multiple antibiotic resistances was determined by the alkaline lysis method. Plasmid curing was also done using acridine orange. In all, 41 different antibiotics resistant pattern was observed. Fifteen isolates showed multiple antibiotic resistances to at least three or more class of antibiotics. Average Percentage values of Antibiotic Resistant Bacteria ranged from 55% -100%. Percentage Antibiotic Resistant values after plasmid curing ranged from 13% -88%. In this study, the various sizes of resistant plasmids and deviation in plasmid profiles ranged from 2.4cm to 2.7cm. This study underscores the wide spread distribution of multiple antibiotic resistant bacteria in carrots and demonstrated that plasmids is one of the important ways to spread and control resistance.


Keywords: Carrot, Antibiotic resistance, Plasmid, Chromosome, Gene.


Citation:

Akoja Sunday O. et al. (2017). Plasmid Profile of Antimicrobial Resistant Bacteria Isolated from Carrot (daucus carota l. Subsp. Sativus) in some selected markets in Ekiti State. Nigeria. J. of Advancement in Medical and Life Sciences. V5I2. DOI: 10.15297/JALS.V5I2.01


Copyright:

© 2017 Akoja Sunday O. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advancement in Medical and Life Sciences