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%20%20%20%20Studies%20on%20Biosurfactants%20Produced%20by%20Hydrocarbon%20Degrading%20Bacteria
Research article
  

Studies on Biosurfactants Produced by Hydrocarbon Degrading Bacteria


A.Mariashobana, K. Nalini and V.Thangapandian

Department of microbiology, Ayya Nadar Janaki Ammal College, Sivakasi.


Corresponding author :

Mariashobana A,
Email:

Received: November 10, 2014,   Accepted: December 7, 2014,   Published:


Abstract:

Petroleum hydrocarbons are the most frequently occurring environmental contaminants because of their extensive use, found in numerous aquatic and terrestrial ecosystems. The present study is aimed in the study of biosurfactants and its role in hydrocarbon degradation. Twenty four bacterial isolates were isolated from soil samples collected from automobile workshops. These isolates were further screened for hydrocarbon degradation and biosurfactant production. After prelimnary screening, nine isolates SA3, SA6, SA7, SA8, SA10, SA12, SA14, SA18 and SA19 were selected for further analysis. Four of the isolates were identified through biochemical screening tests, and all the nine isolates were assessed for biosurfactant production through three screening tests: drop collapsing test, oil spreading technique and emulsification index tests. These screening procedures confirmed that SA3 and SA19 are the efficient strains for biosurfactant production. The presence of glycolipid and rhamnolipid biosurfactants is further supported by the results of TLC, SDS PAGE and FTIR analysis. These two strains were identified through 16SrRNA gene sequencing. Thus the present study made an attempt to isolate biosurfactants from bacteria that can be applied for degrading the petroleum hydrocarbon pollutants deposited in aquatic, marine or terrestrial habitats.


Keywords: Glycolipids, Rhamnolipids, Biosurfactants, Orcinol, Hydrocarbon.


Citation:

Mariashobana A, (2014) Studies on Biosurfactants Produced by Hydrocarbon Degrading Bacteria. J. of Advanced Botany and Zoology, V2I2. DOI: 10.15297/JABZ.V2I2.02


Copyright:

© 2014 Mariashobana A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advanced Botany and Zoology