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%20%20%20%20Screening%20of%20some%20Cameroonian%20Plants%20for%20their%20Potential%20Antibacterial%20Activity%20against%20Human%20Pathogenic%20Bacteria
Research article
  

Screening of some Cameroonian Plants for their Potential Antibacterial Activity against Human Pathogenic Bacteria


Tsobou Roger, Mapongmetsem Pierre Marie, Tane Pierre, Voukeng Kenfack Igor4 Tatsimo Ndendoung Simplice Joel and Van Damme Patrick

1Department of Plant Biology, University of Dschang, Faculty of Science P.O.Box 67 Dschang, Cameroon; Department of Biological Sciences, University of Ngaoundéré, P.O.Box 454 Ngaoundéré, Cameroon.
2Department of Biological Sciences, University of Ngaoundéré, P.O.Box 454 Ngaoundéré, Cameroon.
3Department of Chemistry, University of Dschang, Faculty of Science P.O. Box 67 Dschang, Cameroon.
4Department of Biochemistry, University of Dschang, Faculty of Science P.O. Box 67 Dschang, Cameroon.
5Department of Chemistry, University of Maroua
6Department of Plant Production, Laboratory of Tropical and Subtropical Agriculture and Ethnobotany, Ghent University, Coupure links 653, B 9000 Ghent, Belgium. Department of Crop Science and Agroforestry, Faculty of Tropical Agrisciences, Czech University of Life Sciences Prague, Kamycka 129, 16521 Prague 6-Suchdol, Czech Republic.


Corresponding author :

Tsobou Roger,
Email:

Received: August 2, 2015,   Accepted: September 29, 2015,   Published:


Abstract:

Medicinal plants are geared towards the development of the news antibiotics and the use of medicinal plants in the treatment of infectious diseases. The aim of this study was to determine the antibacterial spectrum of ten Cameroonian medicinal plants traditionally used in treatment of typhoid fever and other infectious diseases. The antibacterial activities of the ten medicinal plants extracts against Salmonella typhi ATCC 6539, S. paratyphi A & S. paratyphi B, Staphylococcus aureus ATCC25922, Enterococcus faecalis ATCC 10541, Escherichia coli ATCC11775, Pseudomonas aeruginosa ATCC 27853 and Klebsiella pneumoniae ATCC 13883 were initially evaluated by well diffusion technique followed by microdilution technique, to determine the zones of inhibition and Minimum inhibition concentration (MIC). The results from the present study have shown that, out of 10 plants investigated, nine plants were found to have antibacterial activity against at least one of the bacteria strains tested with inhibition zones ranging from 7.5 to 24 mm at concentration of 40 mg/ml, 80 mg/ml and 160 mg/ml; with good effect at 160 mg/ml. Enterococcus faecalis was the most susceptible bacteria with 8 plant extracts inhibiting its growth. In comparison, seven plant extracts inhibited the growth of Staphylococcus aureus and Escherichia coli respectively, six plant extracts inhibited the growth of Salmonella typhi, while Pseudomonas aeruginosa was sensitive to five plant extracts, S. paratyphi B was sensitive to four plant extracts, whereas, three plant extracts inhibited the growth of S. paratyphi A and Klebsiella pneumoniae respectively. The highest zones of inhibition diameter were obtained from Senna alata with diameter of 24, 23.5, 22.5, 19, 18.5 and 16.5 mm against Salmonella paratyphi A, Enterococcus faecalis, S. paratyphi B, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli respectively at 160 mg/ml; and from Pseudarthria confertiflora with diameter of 23.5 and 17 mm against S. typhi and Pseudomonas aeruginosa respectively at the same concentration. The lowest MIC values 256 µg/ml was exhibited by the extract of Pseudarthria confertiflora against Klebsiella pneumoniae and the lowest MBC was 1024 µg/ml exhibited by the extracts from Carica papaya against Enterococcus faecalis. Senna alata was particularly effective antibacterial agent being capable of inhibiting the growth of all eight bacteria. Bidens pilosa, Carica papaya and Pseudarthia confertiflora were also good antibacterial agents, each being capable of inhibiting the growth of the majority of bacteria tested. These plants could serves as a cheap source of antibacterial constituent for the treatment of infectious diseases.


Keywords: Medicinal plants, infectious diseases, antibacterial


Citation:

Tsobou Roger et al (2015). Screening of some Cameroonian Plants for their Potential Antibacterial Activity against Human Pathogenic Bacteria.. J. of Advanced Botany and Zoology, V3I3.DOI: 10.5281/zenodo.1000386


Copyright:

© 2015 Tsobou Roger. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advanced Botany and Zoology