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%20%20%20%20Sex%20identification%20of%20Iraqi%20local%20cattle%20using%20PCR-RFLP%20technique
Research article
  

Sex identification of Iraqi local cattle using PCR-RFLP technique


Zainab S. Al-Allak, Maytham A. Dragh, Adnan B. Al-Hawash, Maiada H. Hashim and Duaa A. Hussain

1 Department of Animal Production, College of Agriculture, Misan University, Misan, Iraq.
2 Department of Biology, College of Science, Misan University, Misan, Iraq.
3 Huazhong University of Science and Technology, Wuhan 430074, China.


Zainab S. Al-Allak,
Tel:+9647716225602,
Email:

Received: June 19, 2017,   Accepted: July 14, 2017,   Published:


Abstract:

This study was conducted in the physiology lab. / department of animal production / college of Agriculture / Misan University and Genetic Engineering lab. / department of biology / college of Science / Misan University. The objective of the study was to identify the sex of bovine embryos using amniotic fluid samples by application of PCR-RFLP assay. Amniotic fluid samples were collected from pregnant uteri of cows brought from the slaughter house (25 samples). DNA was extracted and PCR-RFLP was done using SRY primers, the products of amplification furtherly restricted by (HINDⅢ) enzyme. The results showed three bands on Agarose gel electrophoresis diagnosed as male samples, while the presence of one band indicated female samples. Amelogenin X was used as internal control which appeared in both sexes but not restricted by the enzyme. These results furtherly confirmed by inspection of embryos anatomy, the results were accurate in 100%. This method will help producers to build a scientific strategy in management with better prediction of sex ratio in the coming season, also prevent slaughter cows pregnant with wanted embryos or vice versa.


Keywords: sex identification, cattle, PCR-RFLP technique


Citation:

Zainab S. Al-Allak et al. (2017). Sex identification of Iraqi local cattle using PCR-RFLP technique. J. of Advancement in Medical and Life Sciences. V5I3. DOI: 10.5281/zenodo.893483


Copyright:

© 2017 Zainab S. Al-Allak. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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