Antibacterial Activity of Papain Hydrolysates of Isoelectrically-isolated Casein and Thermoprecipitated Alpha-Lactalbumin from Bovine and Caprine Milk on Diarrheagenic Bacteria
Corresponding Author : Timothy Omara
Department of Chemistry, Faculty of Science, Kyambogo University, Kampala-Uganda
Email ID : firstname.lastname@example.org
Received : 2019-01-08 Accepted : 2019-03-01 Published : 2019-03-01
Abstract : The study compared antibacterial potential of hydrolysates of casein and alpha-lactalbumin from cow and goat milk on diarrhea-causing E. coli and S. aureus. Milk samples were skimmed; casein was isoelectrically isolated and alpha-lactalbumin was isolated by filtrate thermoprecipitation at 75°C. 50% of each isolate was buffered and hydrolyzed with papain at 55°C for 2 hours. The degree of hydrolysis of the isolates were monitored in the 2 hours. The hydrolysates were heated to 75°C to inactivate papain, cooled and their bioactivity determined by disc diffusion method. Results showed that alpha-lactalbumins had higher degrees of hydrolysis and bioactivity than caseins; goat alpha-lactalbumin had the highest bioactivity with inhibition zones of 19.6±0.33mm and 19.50±0.29mm on E. coli and S. aureus. Cow lactalbumin inhibited E. coli more than S. aureus with zones of 16.8±0.17mm and 12.50±0.29mm. Cow and goat milk casein hydrolysates inhibited E. coli with inhibition zones of 8.00±0.06mm and 10.90±0.06mm and inhibited S. aureus with inhibition zones of 4.13±0.09mm and 1.9±0.06mm. The hydrolysates can be a source of antibiotics for diarrhea treatment. Research should be done to establish the peptide fractions associated with the observed bioactivity.
Keywords : Assay, diarrhea, isolate, hydrolysis, proteins, inhibition zone.
Citation : Timothy Omara et al (2019). Antibacterial Activity of Papain Hydrolysates of Isoelectrically-isolated Casein and Thermoprecipitated Alpha-Lactalbumin from Bovine and Caprine Milk on Diarrheagenic Bacteria, J. of Advancement in Medical and Life Sciences. V7I3.01. DOI : 10.5281/zenodo.2580653
Copyright : © 2019 Timothy Omara . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Journal of Advancement in Medical and Life Sciences
ISSN : 2348-2931
Volume 7 / Issue 3
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