Email-ID
Password
Forget password?






New User Registration
A Comparative Investigation of Antimicrobial Activities of Proteins Derived From Anodonta Rubens
Research article
  

A Comparative Investigation of Antimicrobial Activities of Proteins Derived From Anodonta Rubens


E. M. ahmed , M. I. Ammar and A.A. Hamdy

1, 3, Chemistry of Natural and Microbial Products, National Research Center, Dokki, Cairo, Egypt, P.O.12622.,
2 Plant Pathology Research Institute; Agriculture Research Center; Giza Egypt.


Corresponding author :

E. M. ahmed,
Email:

Received: February 2, 2014,   Accepted: April 13, 2015,   Published: April 13, 2015.


Abstract:

Protein extracted from Anodonta rubens (Mollusca) was evaluated to investigate in vitro the anti-microbial and the antiviral activities. The crude protein was fractionated by precipitation by different methods. Different measures were determined such as inhibition zone (mm), MIC (minimum inhibitory concentration), EC50. The toxicity of the most active fractions was measured by the XTT assay then CC50 and selective index value were calculated. Amino acid analysis was performed for the purified fraction. Some of the tested protein showed activities with various degrees. The most potential antiviral activity, about 96%, was achieved in case of 75-90% saturation of Polyethylene glycol of molecular weight 4000 (PEG 4000). The protein fractions showed low toxicity. The fraction is rich in essential amino acids, alanine, arginine, glutamine, glycine and phenylalanine. To the best of our knowledge, no similar works were reported with this organism and these compounds.


Keywords: Anodonta rubens, antiviral and antimicrobial. Protein fractions


Citation:

E. M. ahmed etal. (2015). A Comparative Investigation of Antimicrobial Activities of Proteins Derived From Anodonta Rubens. J. of Bioprocessing and Chemical Engineering. V2I4. DOI: 10.15297/JBCE.V2I4.02


Copyright:

© 2015 E. M. ahmed. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
Download PDF

      Journal of Bioprocessing and Chemical Engineering