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Evolutionary Analysis of Regulatory Sequences in Promoter of Senecence-induced Glutamine Synthetase cytosolic isozyme GS1;5 in Arabidopsis thaliana
Research article
  

Evolutionary Analysis of Regulatory Sequences in Promoter of Senecence-induced Glutamine Synthetase cytosolic isozyme GS1;5 in Arabidopsis thaliana


Mamoona Rauf. Muhammad Arif, Aziz Ud Din, Saima Rauf, Muhammad Waqar Hameed, Hussan Ara Begum

1Department of Botany, Garden Campus, Abdul Wali Khan University Mardan, 23200. Pakistan.
2Department of Biotechnology, Garden Campus, Abdul Wali Khan University Mardan, 23200. Pakistan
3Department of Genetics, Garden Campus, Hazara University, Mansehra, Pakistan
4Institute of Agricultural Sciences, New Campus, University of the Punjab, Lahore, Pakistan
5Internationa Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan


Corresponding author :

Mamoona Rauf,
Email:

Received: February 12, 2016,   Accepted: June 27, 2016,   Published:


Abstract:

Glutamine synthetase (GS; EC 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) catalyzes the ATP-dependent addition of ammonium (NH4 +) to the γ-carboxyl group of glutamate and produces glutamine. The enzyme is the product of multiple genes with complex promoters that ensure the transcriptional regulation of the genes in an organ‐ and tissue‐specific manner and in response to a number of environmental variables affecting the nutritional status of the plant cell. In the present study we tested for the presence of highly conserved non-coding sequences (CNSs) in the 1000-bp promoter region by analyzing upstream promoter sequences (counted from the translation initiation codon), of Glutamine Synthetase (GS1;5) and its orthologous genes. A comparative genome-wide bioinformatic analysis performed for identification of evolutionarily preserved regulatory sequences has revealed highly conserved upstream non-coding sequences (CNSs) within 1000-bp promoter region by analyzing upstream promoter sequences (counted from the translation initiation codon), of GS1;5 from Arabidopsis thaliana and its orthologous genes in various plant species. Two consensus sequences are predicted by sequence logo at the position of -880 bp to -890 bp (CNS1) and -522 bp to -549 bp (CNS2) of Arabidopsis promoter region counted from the ATG. Thus identified putative cis-regulatory elements in the promoter region of GS1;5 are expected to allow physical binding of upstream regulatory proteins which are yet to be known, but controlling the senescence-dependent expression of this gene at promoter level in major plant species, under influence of environmental cues to initiate nitrogen remobilization from source to sink.


Keywords: glutamine synthetase, Conserved Regulatory Sequences, Orthologues


Citation:

Mamoona Rauf et al. (2016). Evolutionary Analysis of Regulatory Sequences in Promoter of Senecence-induced Glutamine Synthetase cytosolic isozyme GS1;5 in . J. of Advancement in Medical and Life Sciences. V4I3 DOI: 10.15297/JALS.V4I3.04


Copyright:

© 2016 Mamoona Rauf. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advancement in Medical and Life Sciences