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Optimization Of Mycelial Growth And Exopolysaccharide Production By Calocybe Indica Using Response Surface Methodology
Research article
  

Optimization Of Mycelial Growth And Exopolysaccharide Production By Calocybe Indica Using Response Surface Methodology


K. T. K. Anandapandian and M. Eyini

1 Department of Microbiology, Sokoto state University, Sokoto, Nigeria
2 Research centre in Botany, Thiagarajar College (Autonomous), Madurai, Tamilnadu, India.


Corresponding author :

K.T K. Anandapandian,
Professor,
Department of Microbiology,
Sokoto state University,
Along Airport road, Sokoto, Nigeria.
Contact no: +2348089797483,
Email:

Received: May 12, 2014,   Accepted: June 2, 2014,   Published:


Abstract:

Response surface methodology (statistical analysis) was applied to optimize the medium composition for the mycelial growth and exopolysaccharide production by Calocybe indica in submerged cultures. Firstly, three significant factors (Glucose, peptone and incubation days) on mycelial growth (g/50ml) and exopolysaccharide yield (mg/50ml) were obtained using central composite design. Subsequently, in order to study the mutual interactions between variables, the effects of these factors were further investigated using three-factor, central composite design and the optimal composition for mycelial growth was (in g/L): Glucose 10, peptone 5, and Incubation was 28 days also, the optimal composition for maximum exopolysaccharide production in Calocybe indica was (in g/L): Glucose 15, peptone 2.5, and incubation 21 days under the optimized medium, respectively.


Keywords: Calocybe indica, optimization, Response surface methodology, Exopolysaccharide, statistical analysis.


Citation:

K.T K. Anandapandian, et al (2014). Optimization Of Mycelial Growth And Exopolysaccharide Production By Calocybe Indica Using Response Surface Methodology. J. of Advancement in Medical and Life Sciences,V1I3. DOI: 10.15297/JALS.V1I3.05


Copyright:

© 2014 K.T K. Anandapandian. . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advancement in Medical and Life Sciences