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Bioactivity of Acetone Extract of Sensevieria Aethiopica (Thunb) Leaf
Research article
  

Bioactivity of Acetone Extract of Sensevieria Aethiopica (Thunb) Leaf


David, O. M. and Afolayan, J. A

1Department of Microbiology, Ekiti State University, Ado-Ekiti, Nigeria.
2Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa


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Received: January 29, 2015,   Accepted: April 25, 2016,   Published:


Abstract:

Otitis is highly prevalent infection caused mainly by bacteria and frequently associated with secondary infections. It may lead to other permanent dysfunction in children, and multidrug-resistance has been reported in the implicated bacterial pathogens. The aim of this study is to evaluate the phytochemicals, toxicity and antibacterial potential of acetone leaf extract of Sansevieria aethiopica (Thunb.) against bacterial pathogens responsible for otitis. The phytochemical analyses of the acetone extract were determined using standard assay methods and the antioxidant activity was assessed using 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and ferric reducing power. Brine shrimp lethality test was used to determine the cytotoxicity effect of the extract. Macrobroth dilution method was used for the determination of minimal inhibitory concentration (MIC). The amount of both phenolics and flavonoid in the extract were 19.69 mg tannic acid equivalent/gram and 7.198 mg quercetin equivalent/gram respectively. Flavonols and proathocyanidins concentrations in the extract were 4.351 mg quercetin equivalent/gram and 1.405 mg catechin equivalent/gram respectively. The extract expressed antibacterial effects on both Gram negative and Gram positive bacteria however the activity was more pronounced on Gram negative organisms. The extract has good antioxidant properties. Although, slightly toxic it has both bacteriostatic and bactericidal effects on the selected bacteria associated with otitis especially Gram negative.


Keywords: Otitis, Sensevieria aethiopica, pathogens, antioxidant, phytochemicals, ear infections, antibacterial.


Citation:

David, O. M et al. (2016). Bioactivity of Acetone Extract of Sensevieria Aethiopica (Thunb) Leaf. J. of Advancement in Medical and Life Sciences. V4I2 DOI: 10.15297/JALS.V4I2.01


Copyright:

© 2016 David, O. M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advancement in Medical and Life Sciences