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Isolation of Neurospora Crassa from Cocoa Farm Soil, Antibacterial and Identification Of Bioactive Compounds in the Extract Using Gas Chromatography- Mass Spectrometer
Research article
  

Isolation of Neurospora Crassa from Cocoa Farm Soil, Antibacterial and Identification Of Bioactive Compounds in the Extract Using Gas Chromatography- Mass Spectrometer


Adewole E, Lajide L

Corresponding author :

Adewole E
Department of Chemical Sciences,
Afe Babalola University,
Ado-Ekiti, Nigeria.

Received: January 4, 2014,   Accepted: January 10, 2014,   Published:


Abstract:

Soil has been a free gift of nature that the creator has giving mankind for exploration, there is a world-wide effort by the pharmaceutical chemists to continue to search for new antibiotics, anti-viral, antiparasitic and host of other novel drugs as a result of the grave consequences posed by the current diseases that have developed resistance to many of the known drugs. Neurospora crassa from Fifty years old cocoa farm soil in Ikota, Nigeria was isolated, cultured to yield maximum bioactive compounds using locally designed and fabricated fermenter and bioactive compounds were extracted in ethyl acetate and purified through column chromatography. The fraction X5 was screened for antibacterial activities using agar well diffusion; it has strong zones of inhibition (mm) against Staphylococcus aureus, Bacillus substillis, and Escherichia coli. The results were better than streptomycin standard. The strong antibacterial activities displayed may be connected to the presence of bioactive compounds revealed by the Gc-Ms and these are: 1H-indene,2,3-dihydro-4-methyl having retention time 5.485, height area 310344 and % total 8.267; 1H-Indene,2,3-dihydro-1,2-dimethyl with retention time 6.375, height area 99678 and % total 3.214 and 1H-indene,1-ethylidene having retention time 8.743, height area 134010 and % total 3.567, Naphthalene having retention time 6.191, % of total 18.907 and peak height 700240. From the research work, the cocoa farm soil is an eye opener for the pharmaceutical chemists where they can beam their search point into for the discovery of new and novel compounds that may have various biological activities.


Keywords: cocoa farm soil fermenter, antibacterial, 1H-indene, 2,3-dihydro-4-methyl, 1H-Indene,2, 3-dihydro-1, 2-dimethyl, 1H-indene,1-ethylidene and Naphthalene.


Citation:

Adewole E(2014) Isolation of Neurospora Crassa from Cocoa Farm Soil, Antibacterial and Identification Of Bioactive Compounds in the Extract Using Gas Chromatography - Mass Spectrometer. J. of Advancement in Medical and Life Sciences. V1I1. DOI: 10.15297/JALS.V1I1.05


Copyright:

© 2014 Adewole E . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advancement in Medical and Life Sciences