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Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae)
Research article
  

Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae)


Koto-te-Nyiwa Ngbolua, Tshitenge T. Bishola, Tshimankinda P. Mpiana, Virima Mudogo, Sha-Tshibey D. Tshibangu, Kabamba N. Ngombe, Elumba G. Ekutsu, Dorothée D. Tshilanda, Zoawe B. Gbolo1, Domaine T. Mwanangombo, Fatiany Pierre Ruphin, Robijaona Baholy

1. Department of Biology, Faculty of Science, University of Kinshasa, P.O. Box 190 Kinshasa XI, Democratic Republic of the Congo.
2. Department of Chemistry, Faculty of Science, University of Kinshasa, P.O. Box 190 Kinshasa XI, Democratic Republic of the Congo.
3. Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212 Kinshasa XI, Democratic Republic of the Congo.
4. Malagasy Institute of Applied Research-lot AVB 77-P.O. Box3833 Avarabohitra Itaosy - 102 Antananarivo – Madagascar.


Corresponding author :

Koto-te-Nyiwa Ngbolua,
Department of Biology, Faculty of Science,
University of Kinshasa,
Kinshasa XI, Democratic Republic of the Congo.
Tel.: +243 81 68 79 527,
Email:

Received: April 2, 2014,   Accepted: April 22, 2014,   Published:


Abstract:

Drepanocytosis is a genetic and neglected disease, endemic in some populations originated from tropical regions. One of the main characteristics of this pathology is the production of a large amount of free radicals, leading to a severe oxidative stress and the consumption of NO by free oxygen radicals, and/or by cell-free plasma heme. The present study evaluated the antisickling and radical scavenging activities of extracts from Garcinia punctata stem bark using Emmel’s test and the DPPH assay. G. punctacta was selected through a combination of ethno-pharmacological and zoo-pharmacognosy approaches. The results showed that methanolic, ethyl acetate and dichloromethane soluble fractions, anthocyanins and organic acids exhibited a significant antisickling and antioxidant activity compared to that of n-hexane soluble fraction, as revealed by both the observed normal biconcave form of sickle erythrocyte (normalization rate > 77%). Ethyl acetate exhibited a drastic decrease of radical DPPH absorbance (ED50 = 0.603 ± 0.028 µg/mL). The chemical screening performed on the plant revealed the presence of anthocyanins and organic acids which were then extracted. Total anthocyanins and organic acids revealed interesting antisickling and antioxidant properties that could justify the integration of Garcinia punctata in Congolese pharmacopoeia for the management of sickle cell disease. Some extracts from this plant species could increase nitric oxide by scavenging free oxygen radicals. For the best our knowledge, Garcinia punctata has not been yet previously reported as antisickling plant in the traditional medicine database of Democratic Republic of the Congo.


Keywords: Sickle cell disease, ethnopharmacology, zoopharmacognosy, Garcinia punctata, anthocyanins, organic acids.


Citation:

Koto-te-Nyiwa Ngbolua, et al (2014) Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae). J. of Advanced Botany and Zoology, V1I2. DOI: 10.15297/JABZ.V1I2.04


Copyright:

© 2014 Koto-te-Nyiwa Ngbolua. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


      
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      Journal of Advanced Botany and Zoology